Scientific Research Projects BITSE

Upotreba cijelog genoma mitohondrijske DNA u oplemenjivanju i konzervacijskoj genetici goveda

Acronym: mitoTAUROmics

Competition title: Istraživački projekti

Funding: Croatian science foundation

Total value: 912 378,00 HRK

Funded amount: 912 378,00 HRK

Start date: 01. 07. 2014.

End date: 30. 06. 2018.

Abstract

The main goal of this proposal is to sequence mitochondrial whole genome (mtWGS) in a 500 to 600 bulls or cows (440 to 540 belonging to Istrian cattle, Busha cattle, Slavonian-Syrmian Podolian cattle and Holstein population in Croatia) in order to: 1). identify polymorphic sites within mtWGS in genetically small populations and to analyse (confirm) the presence of selection for the whole mitochondrial genome as well as for each single gene (comparison of non-synonymous versus synonymous mutations); 2). Identify potentially detrimental mutations and to estimate detrimental load in all analysed populations; 3.) develop computerised approach (set of algorithms) that will, in terms of probability or likelihood, enable imputation of sequenced mtWGS haplotypes for other pedigree members as well as to point to animals that are wrongly assigned to the pedigree, 4.) Identify mutations that are associated with phenotypic variability of milk production and fertility traits and to estimate the magnitude of the mtWGS effects (haplotypes) in terms of variance components and 5.) Identify private (specific) mutations that can be used in traceability or genetic bar-coding of species and breeds (this is of particular interest for endangered populations). In performing these comprehensive analyses we will use newly developed methodologies of high-throughput sequencing and complex computing analyses. We believe that this research will stimulate other researchers involved in animal genetics and breeding of other species (dog, horse, swine, rabbit, etc…) for similar researches. Furthermore, results of the project will be economically utilised in cattle breeding as well as conservation management of endangered Croatian breeds. At the same time, we hope that innovative and technologically advanced component of this research will result in good publication record for all scientists involved which will further increase our competitiveness to approach to EU funded projects.

Web: http://mitotauromics.agr.hr/

Leaders

Prof. Ino Čurik, PhD
University of Zagreb Faculty of Agriculture

Associates

Assist. Prof. Dragica Šalamon, PhD
University of Zagreb Faculty of Agriculture, Sveučilište u Zagrebu Agronomski fakultet

Dinko Novosel, dr. med. vet.
Sveučilište u Zagrebu Agronomski fakultet

Vladimir Brajković, mag. ing. agr.
Sveučilište u Zagrebu Agronomski fakultet

Assoc. Prof. Antun Kostelić, PhD
University of Zagreb Faculty of Agriculture

Assoc. Prof. Vlatka Čubrić Čurik, PhD
University of Zagreb Faculty of Agriculture

Assist. Prof. Maja Ferenčaković, PhD
University of Zagreb Faculty of Agriculture